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KMID : 0624620130460010031
BMB Reports
2013 Volume.46 No. 1 p.31 ~ p.36
Over-expression of OsHsfA7 enhanced salt and drought tolerance in transgenic rice
Liu Ai-Ling

Zou Jie
Liu Cui-Fang
Zhou Xiao-Yun
Zhang Xian-Wen
Luo Guang-Yu
Chen Xin-Bo
Abstract
Heat shock proteins play an important role in plant stress tolerance and are mainly regulated by heat shock transcription factors (Hsfs). In this study, we generated transgenic rice over-expressing OsHsfA7 and carried out morphological observation and stress tolerance assays.Transgenic plants exhibited less, shorter lateral roots and root hair. Under salt treatment, over-expressing OsHsfA7 rice showed alleviative appearance of damage symptoms and higher survival rate, leaf electrical conductivity and malondialdehyde content of transgenic plants were lower than those of wild type plants. Meanwhile, transgenic rice seedlings restored normal growth but wild type plants could not be rescued after drought and re-watering treatment. These findings indicate that over-expression of OsHsfA7 gene can increase tolerance to salt and droughtstresses in rice seedlings.
KEYWORD
Drought tolerance, OsHsfA7, Rice, Root morphology, Salt tolerance
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